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Though Amendola (knee) was present at the start of Wednesday's walk-through practice, per ESPN's Mike Reiss, the wideout was officially listed as a non-participant.
Amendola has been diagnosed with a sprained knee and is considered week-to-week, CSN New England reports.
Amendola was forced out of Monday's game against the Bills in the third quarter with a knee issue.
Amendola's teammate, Julian Edelman, is slated to undergo surgery to repair a broken bone in his left foot, ESPN.com reports.
Per ESPN's Mike Reiss, Amendola was on the field for 40 of 80 snaps on offense in Sunday's win over Washington, while catching four passes for 24 yards on five targets.
Amendola caught eight of nine targets for 86 yards and a touchdown in Sunday's 30-23 win over the Jets.
Amendola caught seven passes for 109 yards on five targets in Sunday's 34-27 win over the Colts.
Amendola, who was on the field for 54-of-81 snaps on offense in Sunday's 51-17 win over the Jaguars, per ESPN's Mike Reiss, caught all five of his targets for 39 yards and a TD in the process.
Amendola was on the field for 36 of the Patriots' 86 snaps on offense in Sunday's win over the Bills, ESPN's Mike Reiss reports. Amendola made one catch in the game, a leaping grab that went for 29 yards.
Amendola caught two passes for 24 yards on three targets in Thursday's 28-21 win over the Steelers.
Amendola's teammate, Brandon LaFell, was placed on the regular-season PUP list Saturday, ESPN's Field Yates reports.